Quantum dot probes for cellular analysis
| Autor: | Zheng You, Bin Wang, Chen Hu, Dahai Ren |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Chemistry
General Chemical Engineering Cellular imaging technology industry and agriculture General Engineering Nanotechnology 02 engineering and technology Cellular level equipment and supplies 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Fluorescence 0104 chemical sciences Analytical Chemistry Immunolabeling In vivo Quantum dot Semiconductor nanocrystals Molecular imaging 0210 nano-technology |
| Zdroj: | Analytical Methods. 9:2621-2632 |
| ISSN: | 1759-9679 1759-9660 |
| Popis: | Highly fluorescent and robust semiconductor nanocrystals (known as quantum dots or QDs) play a pivotal role in biological applications. In particular, the excellent optical properties of QDs make them more suitable for immunolabeling, molecular imaging, and multiplexed biological detection for cellular analysis than conventional fluorescent dyes. Many studies have applied QD probes for in vitro and in vivo assays, showing great improvements with respect to gaining insight into bioanalytical chemistry, target specificity, and cytotoxicity. In this review, we discuss the optical properties, specificity, and cytotoxic effects of QDs as well as the progress achieved in multicolor cellular imaging, immunolabeling for signaling pathways, and molecular detection at the cellular level. In addition, carbon QDs as alternatives to the toxic cadmium-based QDs and their applications in biotechnology are discussed. Despite the rapid development and recent progress of QD probes, much more work is required to determine the toxicity of cadmium-containing QDs used in live cells and animals. |
| Databáze: | OpenAIRE |
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