| Autor: |
M.A. Tyumentseva, V.G. Akimkin, Y.V. Mikhaylova, Y.G. Yanushevich, A.I. Tyumentsev, A.A. Shelenkov |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Сборник материалов. |
| DOI: |
10.36233/978-5-9900432-9-9-98 |
| Popis: |
In this study, we assessed the efficiency and off-target activity of the CRISPR/CAS complex with one of the selected guide RNAs using the CIRCLE-seq technology. The gene encoding the human chemokine receptor CCR5 was used as a target sequence for genome editing. The results of this experiment indicate the correct choice of the guide RNA and efficient work of the CRISPR- CAS ribonucleoprotein complex used. CIRCLE-seq technology has shown high sensitivity compared to bioinformatic methods for predicting off-target activity of CRISPR/CAS complexes. We plan to evaluate the efficiency and off-target activity of CRISPR/CAS ribonucleoprotein complexes with other guide RNAs by slightly adjusting the CIRCLE-seq-technology protocol in order to reduce nonspecific DNA breaks and increase the number of reliable reads. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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