Extraction and Fractionation Effects on Antiplasmodial Activity and Phytochemical Composition of Palicourea hoffmannseggiana
| Autor: | Luciano Ferreira Margalho, Maria Fernanda Alves do Nascimento, Douglas Costa Gontijo, Alaíde Braga de Oliveira, Leticia Hiromi Ohashi, Geraldo Célio Brandão |
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| Rok vydání: | 2021 |
| Předmět: |
Rubiaceae
biology Traditional medicine 010405 organic chemistry Palicourea 010401 analytical chemistry Extraction (chemistry) Plasmodium falciparum Fractionation biology.organism_classification 01 natural sciences 0104 chemical sciences chemistry.chemical_compound chemistry Phytochemical composition Parasite hosting Growth inhibition |
| Zdroj: | Planta Medica International Open. 8:e34-e42 |
| ISSN: | 2509-6656 2509-9264 |
| DOI: | 10.1055/a-1375-6456 |
| Popis: | The present study on Palicourea hoffmannseggiana, which was collected at Marapanim, state of Pará, Brazil, comprises the preparation of different stem and leaf extracts and fractions. Ethanol, hydroethanol, and water extracts were prepared by several methods and evaluated for in vitro activity against resistant Plasmodium falciparum (W2 strain), disclosing a low parasite growth inhibition effect (< 50%). Dereplication by UPLC-DAD-ESI−MS of the leaf ethanol extract showed the presence of two known alkaloids, lyalosidic and strictosidinic acids, along with a sinapoyl ester of lyalosidic acid, with m/z 719.33 [M+H]+, which is possibly a new monoterpene indole alkaloid representative. Sequential liquid-liquid acid-base alkaloid separations from the leaf ethanol extract as well as directly from leaf powder afforded fractions of increased parasite growth inhibition, reaching up to 92.5±0.7%. The most bioactive fractions were shown to contain the β-carboline alkaloids harmane and 4-methyl-β-carboline, along with N-methyl-tryptamine and N-acetyl-tryptamine, while monoterpene indole alkaloids were detected in inactive fractions of these processes. The present results demonstrate that these preliminary fractionation methods can lead to significantly active fractions supporting an adequate scale-up to carrying out the isolation of anti-plasmodial compounds. |
| Databáze: | OpenAIRE |
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