Characterization of a Cell Line Derived from Inconnu

Autor:	Jill E. Follett, Mary K. Schmitt
Rok vydání:	1990
Předmět:	Genetics Stenodus leucichthys biology Cell culture Optimum growth Centromere Doubling time Karyotype Embryonic Tissue Aquatic Science biology.organism_classification Molecular biology Fetal bovine serum
Zdroj:	Journal of Aquatic Animal Health. 2:61-67
ISSN:	1548-8667 0899-7659
DOI:	10.1577/1548-8667(1990)002<0061:coacld>2.3.co;2
Popis:	Little is known about the diseases of the northern piscivorous salmonid inconnu Stenodus leucichthys (also known as sheefish). Fish health concerns surrounding transport and culture led to initiation of a cell line, now designated INEM-1, from inconnu embryonic tissue, to be used primarily for viral testing of inconnu. Cells were cultured at 16°C in Eagle's modified minimum essential medium with 10% fetal bovine serum. The fibroblastlike cells have now been passaged 57 times. Optimum growth occurs at 20°C, and doubling time is 1.5 d. Good growth also occurred at 16 and 24°C, but 30°C was rapidly lethal. Optimum density was 100,000 cells/ 35-mm-diameter plate, or 11,000 cells/cm2. Chromosome analysis revealed a modal number of 76 chromosomes, two more than has been reported for blastulas of inconnu. The karyotype consists of 16 metacentric, 8 submetacentric, and 52 acrocentric chromosomes. Nucleolar organizing regions were identified on one chromosome pair. The INEM-1 cell line is susceptible to i...
Databáze:	OpenAIRE
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