Bacterially Synthesized EBNA as a Reagent for Enzyme-Linked Immunosorbent Assays
| Autor: | Gary S. Hayward, S. D. Hayward, Alan L. Scott, S. C. Hartman, Gregory Milman, D. K. Ades, M S Cho |
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| Rok vydání: | 1985 |
| Předmět: |
chemistry.chemical_classification
biology medicine.diagnostic_test Chemistry viruses Antibody titer virus diseases Immunofluorescence medicine.disease Molecular biology Serology chemistry.chemical_compound Enzyme Nasopharyngeal carcinoma immune system diseases hemic and lymphatic diseases biology.protein medicine Antibody DNA Binding domain |
| Zdroj: | Epstein-Barr Virus and Associated Diseases ISBN: 9781461296416 |
| DOI: | 10.1007/978-1-4613-2625-0_39 |
| Popis: | We have synthesized the oarboxy-terminal one third of the EBNA protein as a fusion polypeptide in bacteria. When inoculated into rabbits the purified 28K EBNA polypeptide elicited antibodies which gave the same immunofluorescence staining patterns on lymphoblastoid cell lines as EBNA-positive human serum. The 28K EBNA polypeptide bound tightly to double-stranded DNA suggesting that the carboxy-terminal portion of EBNA is a DNA binding domain. The bacterially synthesized EBNA polypeptide was also employed in an enzyme-linked immunosorbent assay (ELISA). The pattern of anti-EBNA antibody titers measured by the ELISA method in sera from normal individuals and from patients with rheumatoid arthritis, Burkitt lymphoma, nasopharyngeal carcinoma and acute infectious mononucleosis was comparable to that observed in previous serological studies which had employed the classical anti-complement immunofluorescence assay (ACIF). However, in contrast to the ACIF assay, the more sensitive ELISA method was able to detect anti-EBNA antibody in acute infectious mononucleosis serum. |
| Databáze: | OpenAIRE |
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