Kinetics of the Action of Na2SeO3 on Bacillus subtilis Growth as Studied by Microcalorimetry

Autor:	Jin-Li Cao, Hui-Gang Liang, Yi Liu, Ru-Ming Zhao, Zi-Niu Yu, Ping Shen, Song-Sheng Qu
Rok vydání:	2010
Předmět:	Isothermal microcalorimetry Chromatography biology Kinetics General Chemistry Bacillus subtilis biology.organism_classification chemistry.chemical_compound chemistry Biochemistry Toxicity Thermochemistry Bioassay IC50 Toxicant
Zdroj:	Chinese Journal of Chemistry. 20:117-122
ISSN:	1001-604X
DOI:	10.1002/cjoc.20020200202
Popis:	Microcalorimetric bioassay for acute cellular toxicity is based on metabolic heat production from cultured cells. The biological response to toxicants is the inhibition of the heat production rate in cells, and toxicity is expressed as the concentration of toxicant that is 50% effective in this inhibition (IC50). In mis paper, the effect of Na2SeO3 on Bacillus subtilis growth was investigated at 37 °C by microcalorimetry. The relationship between growth rate constants (k) and concentration of Na2SeO3 (c) shows a logarithmic normal distribution, and lC50 is 20.3 μg/mL. All these thermokinetic information is readily obtained by an LKB 2277–204 heat conduction microcalorimeter. Microcalorimetry is a quantitative, inexpensive, and versatile method for toxicology research.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5d1e64b582ac8bfff4a3b935ee9483bb https://doi.org/10.1002/cjoc.20020200202 Zobrazit plný text záznamu Plný text