PINK1 gene mutation by pair truncated sgRNA/Cas9-D10A in cynomolgus monkeys
| Autor: | Li Yan, Yu Kang, Chengzu Long, Xue-Ying Gu, Bin Shen, Zhenzhen Chen, Da-Long Zhi, Jianying Wang, Qiaoyan Yang, Yuyu Niu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
Mutation Ecology Neurodegeneration Mutant Biology medicine.disease medicine.disease_cause Frameshift mutation Cell biology 03 medical and health sciences Exon 0302 clinical medicine Knockout mouse medicine Animal Science and Zoology Guide RNA 030217 neurology & neurosurgery Ecology Evolution Behavior and Systematics 030304 developmental biology Subgenomic mRNA |
| Zdroj: | Zoological Research. 42:469-477 |
| ISSN: | 2095-8137 |
| DOI: | 10.24272/j.issn.2095-8137.2021.023 |
| Popis: | Mutations of PTEN-induced kinase I (PINK1) cause early-onset Parkinson’s disease (PD) with selective neurodegeneration in humans. However, current PINK1 knockout mouse and pig models are unable to recapitulate the typical neurodegenerative phenotypes observed in PD patients. This suggests that generating PINK1 disease models in non-human primates (NHPs) that are close to humans is essential to investigate the unique function of PINK1 in primate brains. Paired single guide RNA (sgRNA)/Cas9-D10A nickases and truncated sgRNA/Cas9, both of which can reduce off-target effects without compromising on-target editing, are two optimized strategies in the CRISPR/Cas9 system for establishing disease animal models. Here, we combined the two strategies and injected Cas9-D10A mRNA and two truncated sgRNAs into one-cell-stage cynomolgus zygotes to target the PINK1 gene. We achieved precise and efficient gene editing of the target site in three newborn cynomolgus monkeys. The frame shift mutations of PINK1 in mutant fibroblasts led to a reduction in mRNA. However, western blotting and immunofluorescence staining confirmed the PINK1 protein levels were comparable to that in wild-type fibroblasts. We further reprogramed mutant fibroblasts into induced pluripotent stem cells (iPSCs), which showed similar ability to differentiate into dopamine (DA) neurons. Taken together, our results showed that co-injection of Cas9-D10A nickase mRNA and sgRNA into one-cell-stage cynomolgus embryos enabled the generation of human disease models in NHPs and target editing by pair truncated sgRNA/Cas9-D10A in PINK1 gene exon 2 did not impact protein expression. |
| Databáze: | OpenAIRE |
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