Evaluation of a new flow cytometry crossmatch procedure for simultaneous detection of cytotoxicity and antibody binding

Autor:	Ann-Charlotte Wikström, Mats Alheim, P. K. Paul, Dan Hauzenberger
Rok vydání:	2013
Předmět:	medicine.diagnostic_test Flow cytometric crossmatch Immunology General Medicine Human leukocyte antigen Biology Antigen binding Biochemistry Molecular biology Complement-dependent cytotoxicity Highly sensitive Flow cytometry IgG binding Genetics medicine Immunology and Allergy Cytotoxicity
Zdroj:	Tissue Antigens. 82:125-130
ISSN:	0001-2815
Popis:	In this study we have evaluated an alternative 96-well format flow cytometry based (FCtox) method which enable simultaneous detection of cytotoxicity and human leukocyte antigen (HLA) antibody binding. Comparable results were obtained in side-by-side comparisons with conventional complement-dependent cytotoxicity (CDC) and flow cytometric crossmatch (FCXM) in terms of sensitivity and specificity. There was 91 and 93% agreement between results obtained by FCtox and CDC for T and B cells, respectively. In addition, comparable results were obtained with FCtox IgG and FCXM IgG for both T and B cells. Furthermore, compared with a recently developed and highly sensitive Luminex based C1q assay we obtained close to 90% method agreement with the FCtox assay. Our alternative cytotoxicity and IgG binding assay which exhibit low intra-and inter-assay variation will improve the workflow and speed up the pre-transplant testing and also allow continuous monitoring of assay performance and proper quality assurance.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5cfecbed3ce914ad38808bb89a72ef8e https://doi.org/10.1111/tan.12151 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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