Aberrant Promoter Methylation of p16 INK4a and O 6 ‐Methylguanine‐DNA Methyltransferase Genes in Workers at a Chinese Uranium Mine

Autor:	Yueping Shen, Lu-jing Yang, Wei Zhang, Jian Tong, Shi-biao Su, Yi Cao, Ya-li Jin
Rok vydání:	2006
Předmět:	Methyltransferase DNA damage Chemistry Public Health Environmental and Occupational Health O-6-methylguanine-DNA methyltransferase Methylation Molecular biology respiratory tract diseases Uranium mine DNA methylation medicine Sputum medicine.symptom neoplasms Gene
Zdroj:	Journal of Occupational Health. 48:261-266
ISSN:	1348-9585 1341-9145
DOI:	10.1539/joh.48.261
Popis:	To find the possible association of gene methylation of p16(INK4a) and O(6)-Methylguanine-DNA Methyltransferase (O(6)-MGMT) with occupational exposure to radon, 91 male miners from a uranium mine in China were divided into 4 groups according to the cumulative doses of radon exposure from 2 to 425 WLM (working-level months), and aberrant promoter methylation of p16(INK4a) and O(6)-MGMT genes in sputum samples was determined by a specific PCR assay. The results revealed that the methylated rates of 16(INK4a) gene (z=2.844, P=0.005) and O(6)-MGMT gene (z=3.034, P=0.002), and the total methylated rate of these two genes (z=3.859, P=0.0001) increased significantly with the cumulative doses of radon among the miners. This methylation could be applied as a potential marker for the detection of early DNA damage induced by occupational radon exposure.
Databáze:	OpenAIRE
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