Cryopreservation of Fighting rooster spermatozoa: effect of different concentrations of glycerol
| Autor: | Andres Moscoso, Diego Galarza, Juan Carlos Alvarado Alvarado, Jorge Xavier Samaniego Campoverde, Daniel Argudo Garzón |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | SPERMOVA. 12:21-26 |
| ISSN: | 2308-4928 2223-9375 |
| DOI: | 10.18548/aspe/0010.04 |
| Popis: | Glycerol (Gly) is the cryoprotective agent (CPA) most widely used to cryopreserve rooster semen. However, its cryoprotection, toxicity, and efficacy may vary in different breeds of roosters (e.g.,fighting rooster). In this sense, this investigation evaluated the effect of different concentrations of Gly added to the Lake-Ravie extender on the kinetic variables and plasma membrane integrity (PMI, equivalent to viability) of rooster spermatozoa. A total of 42 semen ejaculates from 6 Spanish fighting roosters (collected in 7 weekly sessions) by dorsal massage technique were used to conform 7-pools. Each pool was divided into 6-aliquots and then 6 treatments were formed according to Gly concentrations: 0 (control), 2% (Gly-2), 4% (Gly-4), 6% (Gly-6), 8% (Gly-8), and 10% (Gly10). The samples were frozen in static liquid nitrogen vapors, and their post-thaw sperm quality was analyzed using the CASA system (SCA-2018®) and Fluorescence (PI). Results after thawing showed that total (MT, %) and progressive (MP, %) motilities were higher (P |
| Databáze: | OpenAIRE |
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