Expression of uterine receptivity markers in natural cycles and in cycles with hormonal substitution: Preliminary results of 7 women with Tubal-Factor infertility
| Autor: | Ivana Oborna, H Fingerova, Ashok Agarwal, M Kubinyiova, R Novotny, P Havelka |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Gynecology
Infertility medicine.medical_specialty business.industry media_common.quotation_subject Obstetrics and Gynecology Estrogen receptor Histology Tubal factor infertility medicine.disease Prolactin Andrology Basal (phylogenetics) Reproductive Medicine medicine business Ovulation Hormone media_common |
| Zdroj: | Fertility and Sterility. 78:S104 |
| ISSN: | 0015-0282 |
| DOI: | 10.1016/s0015-0282(02)03655-5 |
| Popis: | CYCLES AND IN CYCLES WITH HORMONAL SUBSTITUTION: PRELIMINARY RESULTS OF 7 WOMEN WITH TUBAL-FACTOR INFERTILITY I Oborna, H Fingerova, Department of Obstetrics & Gynecology, Faculty of Medicine, Palacky University Olomouc, R Novotny, P Havelka, M Kubinyiova, Department of Histology & Microscopic Methods, Faculty of Medicine, Palacky University Olomouc, Ashok Agarwal, Cleveland Clinic Foundation Objective: Uterine receptivity plays a major role in normal embryo implantation and successful pregnancy. Recent studies have suggested that implantation occurs in a narrow window of endometrial receptivity between 5 and 7 days after ovulation. However, the role of uterine receptivity in human infertility remains unclear. In this study, we report our preliminary results of pinopode expression and levels of estrogen receptors (ER) and progesterone receptors (PR) in natural cycles and in cycles after standardized hormonal substitution used for cryo-embryo transfer in a group of women with tubal-factor infertility. Design: Prospective study Materials/Methods: The study was approved by the University Ethical Committee, and a written consent was obtained from all participants. All women had a history of infertility of more than 12 months. Infertility evaluation revealed a tubal factor. All women were less than 40 years and had normal body mass index and normal basal serum FSH, LH and prolactin. Normal ovulation (in natural cycles) was confirmed by serum LH surge and ultrasound examination. Endometrial biopsies were performed on days 5 and 7 after ovulation in natural cycles. Hormonal substitution was started in the first day of next cycle using progressively increasing doses of estradiol-valerate (2 mg/d from day 1 to 6, 4 mg/d from day 7 to 10 and 6 mg/d from day 11 to 15). When endometrial thickness reached 8 mm or more, progesterone (P) was added in a dose of 600 mg/d and the dose of estradiol-valerate was decreased to 4 mg/d. Endometrial biopsies were performed on days 5 and 7 after addition of P. Pinopode expressions were determined by scanning electron microscopy. The presence of ER & PR in the endometrial epithelial surface (EES), stroma (ES) and glands (EG) was determined by immunohistochemistry. Results: Study parameters are shown in the table. Conclusions: Our preliminary results indicate no significant difference in the expression of pinpodes in natural versus substituted cycles. Levels of day 5 ER and PR in natural cycles were not significantly different from substituted cycles except for PR in glands and ER in stroma which were significantly higher in the later. Levels of day 7 PR in stroma cells were significantly higher in substituted cycles. Levels of day 7 ER in glands and EES were higher in substituted cycles but the differences did not reach statistical significance. Our observations indicate that hormonal substitution in normally ovulating women may enhance uterine receptivity by mechanisms that involve increased levels of endometrial ER and PR. Supported by: Grant IGA NH6611-3/2001 |
| Databáze: | OpenAIRE |
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