Development of Potentiometric Biosensors Using Electrodeposited Polytyramine as the Enzyme Immobilization Matrix

Autor:	Donald Barnett, J. Justin Gooding, Manihar Situmorang, D. Brynn Hibbert
Rok vydání:	2001
Předmět:	Immobilized enzyme fungi Inorganic chemistry Potentiometric titration technology industry and agriculture Enzyme electrode food and beverages Analytical Chemistry Matrix (chemical analysis) chemistry.chemical_compound chemistry Electrochemistry Malic acid Biosensor Carbodiimide Electrode potential
Zdroj:	Electroanalysis. 13:1469-1474
ISSN:	1521-4109 1040-0397
DOI:	10.1002/1521-4109(200112)13:18<1469::aid-elan1469>3.0.co;2-u
Popis:	The application of electrodeposited polytyramine to the fabrication of potentiometric enzyme electrodes is described. Polytyramine and the enzyme malic acid dehydrogenase were co-deposited onto a pH-sensitive tungsten electrode. The entrapped enzyme was subsequently covalently attached to the polymer film using carbodiimide coupling. Malic acid biosensors fabricated by this method were highly reproducible with good stability, linear detection range between 0.1 and 3.5 mM malic acid and the lowest concentration of malic acid detected was 0.1 mM. Because the acid produced in the enzyme reaction titrates the buffer, the relationship between malic acid concentration and electrode potential is not Nernstian but can be empirically calibrated as a logit function. Electrodeposited polytyramine produces reproducible and stable enzyme electrodes.
Databáze:	OpenAIRE
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