Microsatellites for Lindera species

Autor:	Craig S. Echt, Dennis L. Deemer, Thomas L. Kubisiak, C.D. Nelson
Rok vydání:	2006
Předmět:	Lindera Genetics Ecology biology Endangered species Locus (genetics) biology.organism_classification Biochemistry General Biochemistry Genetics and Molecular Biology Loss of heterozygosity Lindera melissifolia Microsatellite Lindera benzoin Allele
Zdroj:	Molecular Ecology Notes. 6:1171-1173
ISSN:	1471-8286 1471-8278
DOI:	10.1111/j.1471-8286.2006.01479.x
Popis:	Microsatellite markers were developed for conservation genetic studies of Lindera melissifolia (pondberry), a federally endangered shrub of southern bottomland ecosystems. Microsatellite sequences were obtained from DNA libraries that were enriched for the (AC) n simple sequence repeat motif. From 35 clone sequences, 20 primer pairs were designed and evaluated. Eleven primer pairs amplified polymorphic marker loci in pondberry while two did so in Lindera benzoin (spicebush). In 46 samples from a single pondberry site the number of microsatellite alleles ranged from two to 11 per locus with observed heterozygosity values of 0.07–0.91.
Databáze:	OpenAIRE
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