Immunohistochemical Analysis of the Expression of Cytokeratins in Acquired Cholesteatoma and Its Clinico-Radiological Correlation
| Autor: | Anupam Kanodia, Aanchal Kakkar, Yash Verma, Diya Roy, Hitesh Verma, Chirom Amit Singh, Rabia Monga, Deepali Jain, Alok Thakar, Kapil Sikka |
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| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Journal of Audiology and Otology. 27:97-103 |
| ISSN: | 2384-1710 2384-1621 |
| Popis: | Background and Objectives: Cholesteatomatous chronic otitis media acquires epithelial proliferation and differentiation characteristics, which render it able to erode the underlying bone and cause complications. We attempt to characterize the cholesteatoma epithelium by observing the expression of cytokeratins (such as 34ße12, CK17, and CK13) and Ki67 among patients with cholesteatoma with different aggressiveness as compared to disease-free controls.Subjects and Methods: In this prospective study (2017-2021), we enrolled all consenting consecutive patients with cholesteatomatous chronic otitis media. They were staged in accordance with the staging guidelines of the European Academy of Otology and Neurotology and the Japanese Otological Society. Bony external auditory canal (EAC) skin specimens of the patients undergoing tympanoplasty were chosen as controls. We did an immunohistochemical analysis of the cholesteatoma specimens and normal bony EAC controls by observing the expression of 34ße12, CK17, CK13, and Ki67 across the layers of the epithelium. Fisher’s exact test and chi-square test were used to evaluate any statistical significance between the cases and the controls, and the subgroups were made based on the clinical stage.Results: An increased expression of CK17 (ppp |
| Databáze: | OpenAIRE |
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