| Popis: |
Publisher Summary Colony hybridization has been carried out using several types of supports. Nylon membranes are the most durable with regard to resistance to damage and repetitive use but are also the most expensive and require many steps similar to those required for nitrocellulose. Nylon membranes from different manufacturers require different treatments and the instructions of the manufacturer must be followed closely for optimal results. In comparison to other hybridization supports, several steps—such as baking in a vacuum oven, prehybridization, and some probe purification steps—can be eliminated by using the labeling procedure described in the chapter. Signals for a perfect match are about l0 to 20 times stronger than those for a singlebase internal mismatch. |
