| Autor: |
Simon Haas, Magdalena Antes, Daniel R. Leonce, Daniel Hübschmann, Tobias Boch, Sergio Triana, Diana Ordoñez-Rueda, Dominik Vonficht, Malte Paulsen, Lea Jopp-Saile, Daniel Nowak, Lars Velten, Theodore Alexandrov, Wolf-Karsten Hofmann, Johann-Christoph Jann, Andreas Trumpp, Pablo Hernández-Malmierca, Carsten Müller-Tidow, Raphael Lutz, Simon Raffel, Vladimir Benes, Beáta Ramasz |
| Rok vydání: |
2021 |
| Předmět: |
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| DOI: |
10.1101/2021.03.18.435922 |
| Popis: |
Single-cell genomics has transformed our understanding of complex cellular systems. However, excessive costs and a lack of strategies for the purification of newly identified cell types impede their functional characterization and large-scale profiling. Here, we have generated high content single-cell proteo-genomic reference maps of human blood and bone marrow that quantitatively link the expression of up to 197 surface markers to cellular identities and biological processes across all major hematopoietic cell types in healthy aging and leukemia. These reference maps enable the automatic design of cost-effective high-throughput cytometry schemes that outperform state-of-the-art approaches, accurately reflect complex topologies of cellular systems, and permit the purification of precisely defined cell states. The systematic integration of cytometry and proteo-genomic data enables measuring the functional capacities of precisely mapped cell states at the single-cell level. Our study serves as an accessible resource and paves the way for a data-driven era in cytometry. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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