Atomic force microscopy analysis of orientation and bending of oligodeoxynucleotides in polypod-like structured DNA
| Autor: | Yuki Takahashi, Tomoko Emura, Mengmeng Tan, Kumi Hidaka, Hiroshi Sugiyama, Makiya Nishikawa, Tomoki Shiomi, Yoshinobu Takakura, Natsuki Takahashi, Masayuki Endo |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Atomic force microscopy
Bending Condensed Matter Physics Atomic and Molecular Physics and Optics Orientation (vector space) chemistry.chemical_compound Crystallography chemistry Dna nanostructures DNA origami Structure–activity relationship General Materials Science Self-assembly Electrical and Electronic Engineering DNA |
| Zdroj: | Nano Research. 8:3764-3771 |
| ISSN: | 1998-0000 1998-0124 |
| DOI: | 10.1007/s12274-015-0875-y |
| Popis: | We previously demonstrated that polypod-like structured DNA, or polypodna, constructed with three or more oligodeoxynucleotides (ODNs), is efficiently taken up by immune cells such as dendritic cells and macrophages, depending on its structural complexity. The ODNs comprising the polypodna should bend to form the polypod-like structure, and may do so by adopting either a bendtype conformation or a cross-type conformation. Here, we tried to elucidate the orientation and bending of ODNs in polypodnas using atomic force microscopy (AFM). We designed two types of pentapodnas (i.e., a polypodna with five pods) using 60- to 88-base ODNs, which were then immobilized on DNA origami frames. AFM imaging showed that the ODNs in the pentapodna adopted bend-type conformations. Tetrapodna and hexapodna also adopted bend-type conformations when they were immobilized on frames under unconstrained conditions. These findings provide useful information toward the coherent design of, and the structure–activity relationships for, a variety of DNA nanostructures. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink