Late Breaking Abstract - Increased serum levels of YKL-40 in male smoking chronic obstructive pulmonary disease patients associated with altered miRNA levels in alveolar macrophages
| Autor: | C. Magnus Sköld, Benedikt Zöhrer, Anna James, Åsa M. Wheelock, Nabil Souihi, Bettina Brundin, SE Dahlen, David J. Erle, Reza Karimi |
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| Rok vydání: | 2020 |
| Předmět: |
musculoskeletal diseases
medicine.medical_specialty COPD Lung Microarray medicine.diagnostic_test business.industry Pulmonary disease respiratory system medicine.disease Gastroenterology CHI3L1 respiratory tract diseases medicine.anatomical_structure Bronchoalveolar lavage Internal medicine microRNA Medicine Risk factor business |
| Zdroj: | Monitoring airway disease. |
| Popis: | Background: The protein YKL-40 (CHI3L1) is associated with smoking and chronic obstructive pulmonary disease (COPD). Smoking is a major risk factor for COPD, and the importance of gender on molecular COPD sub-phenotypes is emerging. Aims and Objectives: To investigate the influence of gender and smoking on YKL-40 levels, and putative regulatory mechanisms of YKL-40 expression in the lung. Methods: YKL-40 was quantified by ELISA in serum and bronchoalveolar lavage (BAL) fluid in never-smokers (n=40), smokers with normal lung function (n=40), as well as smokers (n=27) and ex-smokers (n=11) with mild to moderate COPD. CHI3L1 mRNA and associated miRNA were quantified in BAL cells and bronchial epithelial cells (BEC) using microarray. Results: Serum YKL-40 was significantly elevated in the male smoking COPD patients compared to male smokers without COPD (p Conclusions: Alveolar macrophages, which make up >85% of BAL cells, could account for the higher YKL-40 serum levels observed in smoking men with COPD, possibly via specific miRNA. This could point towards a male-dominated COPD sub-phenotype associated with chitinase alterations. |
| Databáze: | OpenAIRE |
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