Sensitive HPTLC method for monitoring dissolution profiles of diclofenac from different tablets containing combined diclofenac and acetaminophen

Autor: Hiten J. Shah, I. S Rathod, Chamanlal J. Shishoo, S. S Savale, Shailesh A. Shah
Rok vydání: 2003
Předmět:
Zdroj: Journal of Planar Chromatography – Modern TLC. 16:36-44
ISSN: 1789-0993
0933-4173
DOI: 10.1556/jpc.16.2003.1.8
Popis: Two fundamentally different analytical methods have been validated and used to monitor release of diclofenac and acetaminophen (paracetamol), from commercially available combination tablet formulations, in dissolution media. A sensitive HPTLC method was developed and validated for analysis of diclofenac. The filtered dissolution samples were directly spotted on to precoated silica gel 60 F 254 TLC plates and diclofenac was separated by use of chloroform-methanol-ammonia solution, 10 + 25 + 0.25 ( v/v ), as mobile phase. The spots were scanned at λ = 277 nm. Peak areas for diclofenac were found to be linearly dependent on the amount applied to the plate in the range 30–390 ng spot −1 ( r = 0.999).For estimation of acetaminophen a simple colorimetric method, involving reaction with sodium nitrite in acid then treatment with alkali to produce the 3-nitroacetaminophane anion (yellow color), was validated and used. The intensity of the yellow color, monitored at λ = 430 nm, was found to be linearly dependent o...
Databáze: OpenAIRE
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