Antiadipogenic Effects of Salvia plebeia R. Br. Extracts by Extraction Conditions in 3T3-L1 Preadipocytes
| Autor: | Soo Im Choi, Hoyoung Kwak, Jae Yoon Kim, Jong Gil Choi, Je-Hyuk Lee |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
chemistry.chemical_classification
Lipoprotein lipase biology Pharmaceutical Science Peroxisome proliferator-activated receptor 3T3-L1 Plant Science biology.organism_classification Biochemistry Genetics and Molecular Biology (miscellaneous) Molecular biology chemistry.chemical_compound Fatty acid synthase chemistry Biochemistry Adipogenesis biology.protein Oil Red O Salvia plebeia Receptor Agronomy and Crop Science |
| Zdroj: | Korean Journal of Medicinal Crop Science. 23:245-252 |
| ISSN: | 2288-0186 1225-9306 |
| DOI: | 10.7783/kjmcs.2015.23.3.245 |
| Popis: | *YD Life Science Research Institutes, YD Life Science Co. Ltd., Seongnam 462-807, Korea.**Department of Food and Nutrition, Kongju National University, Yesan 340-802, Korea.ABSTRACT : This study was carried out to investigate the effects of Salvia plebeia R. Br. ethanolic extract with differentaspects (stem/leaf and whole plant) on differentiation and lipid accumulation in 3T3-L1 preadipocytes. The morphologicalchanges and the degrees of lipid accumulation in 3T3-L1 cells were measured by Oil Red O staining and intra-cellular trig-lyceride (TG) assay. The mRNA expressions of special peroxisome proliferation activated receptor- genes (PPAR), CCAAT/enhancer-binding protein (C/EBPα), fatty acid synthase (FAS) and lipoprotein lipase (LPL) were detected by reverse tran-scriptase polymerase chain reaction (RT-PCR). The 50% ethanolic extracts (100μg/mL) of stem and leaf (SALE) and 30%ethanolic extracts (100 g/mL) of whole plant (SAE) from Salvia plebeia R. Br. were significantly attenuated lipid accumula-tion during adipogenesis in 3T3-L1 cells. Ethyl acetate-soluble fractions (50μg/mL) significantly inhibited lipid dropletaccumulation in 3T3-L1 cells. In addition, SALE induced down-regulation of specific adipogenic transcriptional factors (C/EBPα and PPARγ) and target genes (FAS and LPL) during adipogenesis. Salvia plebeia R. Br. may be used as a safe and effi-cient natural substance to manage obesity.Key Words : Salvia plebeia, 3T3-L1, Adipogenesis, C/EBP α, PPARγ, FAS, LPL |
| Databáze: | OpenAIRE |
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