Fabrication of Micro Flow Cell for Multi-Analyte Sandwich Immunoassay Using Teflon Micro Check Valves

Autor:	Hirotaka Sato, Y. Ashihara, K. Akahori, M. Isomura, Shuichi Shoji, N. Honda
Rok vydání:	2001
Předmět:	Fabrication business.product_category Materials science Check valve medicine.diagnostic_test Pressure control Analytical chemistry Flow cell Reagent Immunoassay medicine Sandwich immunoassay business Multi analyte Biomedical engineering
Zdroj:	Micro Total Analysis Systems 2001 ISBN: 9789401038935
DOI:	10.1007/978-94-010-1015-3_177
Popis:	A micro flow cell for sandwich immunoassay using Teflon (Asahi Glass Co.: CYTOP™) check valves for sample and reagent inlets is designed and fabricated. The check valves are used not only to prevent reverse flow of injected solutions but also to realize sequential injection of the sample and reagent by simple applied pressure control. The valve structure is designed to control the width and thickness of the sample. We examined a fluorescent sandwich immunoassay for human AFP (α-fetoprotein) as a preliminary experiment and fast immunoassay within 30 minutes compared to the conventional one was realized in the micro flow cell.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5c863bdee4be2b385d04b8ede049db14 https://doi.org/10.1007/978-94-010-1015-3_177 Zobrazit plný text záznamu