Sequences of stilboflavin C: towards the peptaibiome of the filamentous fungus Stilbella (= Trichoderma) flavipes
| Autor: | Lutz Götze, Andreas Vilcinskas, Hans Brückner, Hans von Döhren, Thomas Degenkolb |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Sequence analysis Peptide 01 natural sciences Biochemistry 03 medical and health sciences Structural Biology Drug Discovery Molecular Biology Peptide sequence Pharmacology chemistry.chemical_classification Fungal protein Hplc analysis biology 010405 organic chemistry Chemistry Organic Chemistry General Medicine biology.organism_classification 0104 chemical sciences Filamentous fungus 030104 developmental biology Trichoderma Proteome Molecular Medicine |
| Zdroj: | Journal of Peptide Science. 22:517-524 |
| ISSN: | 1075-2617 |
| Popis: | Filamentous fungi of the genus Stilbella are recognized as an abundant source of naturally occurring α-aminoisobutyric acid-containing peptides. The culture broth of Stilbella (Trichoderma) flavipes CBS 146.81 yielded a mixture of peptides named stilboflavins (SF), and these were isolated and separated by preparative TLC into groups named SF-A, SF-B, and SF-C. Although all three of these groups resolved as single spots on thin-layer chromatograms, HPLC analysis revealed that each of the groups represents very microheterogeneous mixtures of closely related peptides. Here, we report on the sequence analysis of SF-C peptides, formerly isolated by preparative TLC. HPLC coupled to QqTOF-ESI-HRMS provided the sequences of 10 16-residue peptides and five 19-residue peptides, all of which were N-terminally acetylated. In contrast to the previously described SF-A and SF-B peptaibols, SF-C peptaibols contain Ser-Alaol or Ser-Leuol, which are rarely found as C-termini, and repetitive Leu-Aib-Gly sequences, which have not been detected in peptaibols before. Taking the previously determined sequences of SF-A and SF-B into account, the entirety of peptides produced by S. flavipes (the 'peptaibiome') approaches or exceeds 100 non-ribosomally biosynthesized peptaibiotics. Copyright © 2016 European Peptide Society and John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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