In Vitro Assessment of the Hepatotoxicity Potential of Therapeutic Oligonucleotides
| Autor: | Sabine Sewing, Tanja Minz, Franziska Boess |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Prioritization 040301 veterinary sciences Oligonucleotide Chemistry In vitro cytotoxicity 04 agricultural and veterinary sciences In vitro 0403 veterinary science 03 medical and health sciences Safety profile 030104 developmental biology In vivo Antisense oligonucleotides Cancer research Cytotoxicity |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493996698 |
| Popis: | The mechanisms of antisense oligonucleotide-induced liver toxicity are still poorly understood. Assessment of the hepatic safety profile is currently mostly investigated directly in rodent studies. A predictive preclinical in vitro model that is capturing liver liabilities of antisense oligonucleotides can be of great help to be used as a first filter in the screening process of therapeutic oligonucleotides. We describe here an in vitro cytotoxicity assay using freshly isolated mouse hepatocytes or cryopreserved human hepatocytes that recapitulates the hepatotoxic profile of antisense oligonucleotides previously observed in rodents and can be used for the prioritization of molecules prior to in vivo testing. |
| Databáze: | OpenAIRE |
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