Studies of infectious bovine rhinotracheitis virus

Autor:	F. J. DePauli, L. R. Sabina
Rok vydání:	1972
Předmět:	chemistry.chemical_classification medicine.medical_specialty Arginine viruses General Medicine Biology Virology Virus Amino acid chemistry.chemical_compound Infectious bovine rhinotracheitis virus Medical microbiology chemistry medicine Single amino acid Leucine DNA
Zdroj:	Archiv f�r die gesamte Virusforschung. 36:380-390
ISSN:	1432-8798 0304-8608
DOI:	10.1007/bf01249869
Popis:	When infectious bovine rhinotracheitis (IBR) virus-infected MDBK cells were overlaid with Eagle's MEM lacking a single amino acid, virus yields were lowered. The omission of either arginine or leucine greatly reduced viral yields; but, further supplementation of MEM medium with either of these amino acids resulted in virus yields higher than controls. The configurations and structures of these two essential amino acids were found to be influential in determining final yields. Radioisotope experiments with both uninfected and virus-infected cultures, demonstrated that the omission of arginine or leucine from the nutrient medium affected incorporation of3H-valine and3H-thymidine into protein and DNA, respectively.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5c712bfc11bf66e616313d3be5290dc4 https://doi.org/10.1007/bf01249869 Zobrazit plný text záznamu Full text from SpringerLink