Regeneration and genetic transformation of Hagenia abyssinica (Bruce) J.F. Gmel. (Rosaceae) with rolB gene

Autor: Legesse Negash, Tileye Feyissa, Margareta Welander, Li-Hua Zhu
Rok vydání: 2007
Předmět:
Zdroj: Plant Cell, Tissue and Organ Culture. 88:277-288
ISSN: 1573-5044
0167-6857
DOI: 10.1007/s11240-006-9200-5
Popis: The effects of growth regulators, wounding and antibiotics on regeneration of Hagenia abyssinica were investigated and the rolB gene was introduced into this species by Agrobacterium-mediated transformation. Regeneration was affected by type of growth regulators, wounding and antibiotics. Up to 100% regeneration could be obtained. Three transformed clones (T1, T2.1, T2.2), confirmed by PCR and Southern blot, were obtained only by excluding kanamycin from the selection medium 6 weeks after culture, followed by selection during shoot multiplication. RT-PCR revealed strong expression of rolB gene in shoots and roots of all the transgenic clones, but from leaf samples, it was detected only in T1. Rooting frequency was 77% (T1), 50% (T2.1), 57% (T2.2) and 0% for control shoots on growth regulator-free rooting medium.
Databáze: OpenAIRE
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