Molecular Cloning, Modeling, and Characterization of Type 2 Metallothionein from Plantago ovata Forsk

Autor:	Shonima Talapatra, Sarmistha Sen Raychaudhuri, Nirmalya Ghoshal, Amitava Moulick, Debashis Mukhopadhyay
Rok vydání:	2013
Předmět:	chemistry.chemical_classification Exon Protein sequencing Biochemistry biology chemistry Metallothionein Homology modeling Molecular cloning biology.organism_classification Gene Plantago ovata Amino acid
Zdroj:	Sequencing. 2013:1-10
ISSN:	2090-0333 2090-0325
Popis:	Plantago ovata Forsk is a medicinally important plant. Metallothioneins are cysteine rich proteins involved in the detoxification of heavy metals. Molecular cloning and modeling of MT from P. ovata is not reported yet. The present investigation will describe the isolation, structure prediction, characterization, and expression under copper stress of type 2 metallothionein (MT2) from this species. The gene of the protein comprises three exons and two introns. The deduced protein sequence contains 81 amino acids with a calculated molecular weight of about 8.1 kDa and a theoretical pI value of 4.77. The transcript level of this protein was increased in response to copper stress. Homology modeling was used to construct a three-dimensional structure of P. ovata MT2. The 3D structure model of P. ovata MT2 will provide a significant clue for further structural and functional study of this protein.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5c4228827246eee5783079f02cf80bf8 https://doi.org/10.1155/2013/756983 Zobrazit plný text záznamu Plný text