Transformation of substrate specificity of EcoRl restrictase by glycerol

Autor:	Tikhonenko Ti, Zavizion Ba, Karamov Ev, B. S. Naroditskii
Rok vydání:	1977
Předmět:	chemistry.chemical_classification biology General Medicine General Biochemistry Genetics and Molecular Biology Restriction fragment chemistry.chemical_compound Hydrolysis Restriction site Restriction enzyme Enzyme chemistry Biochemistry Ionic strength biology.protein Glycerol DNA
Zdroj:	Bulletin of Experimental Biology and Medicine. 84:973-975
ISSN:	1573-8221 0007-4888
DOI:	10.1007/bf00798520
Popis:	In the presence of glycerol, EcoRl restriction endonuclease hydrolyzes DNA into a larger number of fragments than under ordinary conditions. On the addition of glycerol to 50% concentration, this enzyme begins to act by what is called the “EcoRl' type” of restriction, which was producible experimentally only by reducing the ionic strength and increasing the pH of the solution. However, under these extremal conditions, the enzyme is quickly inactivated and does not give reproducible results, especially during the hydrolysis of high-molecular-weight DNA. The conditions found for manifestation of “EcoRl' activity” yield reproductible results, which is essentially equivalent to the discovery of a new “restriction endonuclease”.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5c3d8014e4115a99afc36d9425a31348 https://doi.org/10.1007/bf00798520 Zobrazit plný text záznamu Full text from SpringerLink