Immunological Activities of Rat Lymphocytes

Autor:	Raynald Roy, Fritz Daguillard, Jean McNicoll
Rok vydání:	1976
Předmět:	biology business.industry medicine.medical_treatment T cell Immunology Spleen General Medicine Epitope Thymectomy medicine.anatomical_structure Antigen biology.protein Immunology and Allergy Medicine Lymph Antibody business Cytotoxicity
Zdroj:	International Archives of Allergy and Immunology. 52:32-41
ISSN:	1423-0097 1018-2438
Popis:	Lymphocytes from the peripheral blood, thoracic duct, spleen and lymph nodes of normal, thymectomized (Tx) and thymectomized lethally irradiated marrow reconstituted (TxBM or B rats) Lewis rats were studied for their ability to proliferate in vitro in the presence of Con A or PHA. At the same time the lymphoid tissues of these animals were examined for the presence of B cells or T cells by immunofluorescence staining with antiserums to rat immunoglobulins or rat brain antigens (ARBS), respectively. The specificity of ARBS fort T cells was first established in both cytotoxicity and immunofluorescence studies with thymocytes as well as purified T and B cells. In various lymphoid tissues from Tx and B rats, cells carrying brain antigens were found which were unable to respond to Con A and PHA. Thymectomy has a lesser effect on the response to Con A than to PHA; this was particularly true in the thoracic duct where, following thymectomy, lymphocytes continued to respond to Con A but lost their ability to respond to PHA. This suggests that the response to PHA and Con A may belong to different cells’ subsets but that the ability to respond to either one of these mitogens may be impaired in cells carrying known T cell markers.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5c2dcfbd67a400091284c3d251edbf2d https://doi.org/10.1159/000231664 Zobrazit plný text záznamu