A new acute leukemia-associated blast cell antigen detected by a monoclonal antibody

Autor:	Bing J. Shi, Paul I. Terasaki, Kathy Lucero, Ronald Billing
Rok vydání:	1982
Předmět:	Acute leukemia Acute myeloblastic leukemia Immunology Myeloid leukemia Cell Biology Hematology Biology medicine.disease Biochemistry Molecular biology Leukemia medicine.anatomical_structure Antigen hemic and lymphatic diseases Myeloblast medicine biology.protein Antibody K562 cells
Zdroj:	Blood. 59:1203-1206
ISSN:	1528-0020 0006-4971
DOI:	10.1182/blood.v59.6.1203.1203
Popis:	A monoclonal mouse antibody has been raised to the common acute lymphoblastic leukemia (cALL) cell line Reh. It is a cytotoxic antibody of the IgG2, subclass that reacts with leukemia cells from the following patients: 69% non-B non-T ALL, 50% T-ALL, 18% acute myeloblastic leukemia (AML), and 66% chronic myeloid leukemia (CML) blast crisis lymphoid cells. Other types of leukemia and all normal blood cells tested were negative, including T and B lymphocytes, granulocytes, monocytes, erythrocytes, and spleen cells. The detected antigen appears to be a type of blast cell antigen because it is also present on phytohemagglutinin (PHA) blast cells, myeloblast from normal bone marrow cells (by CFU-C), and all lymphoblastoid cell lines tested. Only one active antibody species could be detected by preparative isoelectric focusing on polyacrylamide gels and by protein-A-Sepharose affinity chromatography.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5c230b967513db6df22b75c58ec152b4 https://doi.org/10.1182/blood.v59.6.1203.1203 Zobrazit plný text záznamu