Pleiotropic Effects in Transgenic Tobacco Plants Expressing the Oryzacystatin I Gene

Autor: Rafael Gutiérrez-Campos, Miguel A. Gómez-Lim, Juan Antonio Torres-Acosta, José de Jesús Pérez-Martínez
Rok vydání: 2001
Předmět:
Zdroj: HortScience. 36:118-119
ISSN: 2327-9834
0018-5345
DOI: 10.21273/hortsci.36.1.118
Popis: A study was undertaken to evaluate possible phenotypic alterations in transgenic tobacco (Nicotiana tabacum L. cv. Xanthi) plants expressing the oryzacystatin I gene. Morphophysiologic parameters, such as growth rate, biomass, and leaf, flower, and fruit characteristics were analyzed. Transgenic plants overexpressing the cysteine proteinase inhibitor showed increased growth rate and dry weight, earlier flowering, and increased numbers of flowers and seeds. These pleiotropic effects were correlated with expression level of oryzacystatin I in transgenic lines. The results suggest that oryzacystatin I disrupts the normal activity of cysteine proteinases, which are involved in important physiological processes. transgenic plants might cause undesirable phenotypic alterations. To address this issue, a study was undertaken to examine the pheno- typic characteristics of transgenic tobacco plants overexpressing the oryzacystatin I gene.
Databáze: OpenAIRE