Impaired Somatodendritic Responses to Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) of Supraoptic Neurones in PACAP type I -Receptor Deficient Mice
| Autor: | André Calas, Izumi Shibuya, Philippe Brabet, Claire-Marie Vacher, Gérard Alonso, H. Widmer, Joël Bockaert, Govindan Dayanithi, Françoise Jamen |
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| Rok vydání: | 2003 |
| Předmět: |
endocrine system
medicine.medical_specialty Vasopressin Endocrine and Autonomic Systems Endocrinology Diabetes and Metabolism Adenylate kinase Neuropeptide Biology Supraoptic nucleus Calcium in biology Cellular and Molecular Neuroscience Pituitary adenylate cyclase-activating peptide Endocrinology Oxytocin Hypothalamus Internal medicine medicine hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | Journal of Neuroendocrinology. 15:871-881 |
| ISSN: | 1365-2826 0953-8194 |
| Popis: | The role of pituitary adenylate cyclase-activating polypeptide (PACAP) type I receptor (PAC1 receptor) in regulating hypothalamic supraoptic neurones was investigated using PAC 1 receptor-deficient male mice (PAC1 -/- ). The effects of PACAP on [Ca 2+ ] i were investigated in freshly dissociated supraoptic neurones and on the somatodendritic release of vasopressin and oxytocin, examined on intact supraoptic nuclei. In supraoptic neurones from wild-type mice (PAC1 +/+ ), 100 nM PACAP induced an increase in [Ca 2+ ] i and release of vasopressin and oxytocin, whereas in heterozygous (PAC1 +/- ) and null-mutant mice (PAC1 -/- ), PACAP was much less effective. PACAP had no effect on these two parameters when applied to isolated neurohypophysial nerve terminals of PAC1 +/+ and PAC1 -/- mice, and rats. In conclusion, the PAC1 receptor is solely responsible for the PACAP-induced [Ca 2+ ] i signalling and secretion of vasopressin and oxytocin in the somatodendritic region of supraoptic neurones. |
| Databáze: | OpenAIRE |
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