Evaluating conditions for producing spray-dried formulations ofAnagrapha falciferanucleopolyhedroviruses (AfMNPV)

Autor:	Robert W. Behle, Michael R. McGuire, Patricia Tamez-Guerra
Rok vydání:	2006
Předmět:	Spray dried biology Alkalinity Raw material biology.organism_classification chemistry.chemical_compound chemistry Insect Science Spray drying Botany Trichoplusia Lignin Food science Anagrapha falcifera Agronomy and Crop Science
Zdroj:	Biocontrol Science and Technology. 16:941-952
ISSN:	1360-0478 0958-3157
DOI:	10.1080/09583150600828312
Popis:	We investigated how altering parameters during the production of spray-dried lignin formulations affected the insecticidal activity of a baculovirus (AfMNPV), which was originally isolated from celery looper, Anagrapha falcifera (Kirby). Exposure to high temperature, varied pH of the dryer feedstock, substitution of formulation ingredients, and different commercial production lots of virus were evaluated for their effect on the insecticidal activity of AfMNPV. Insecticidal activities of treatments were determined by droplet-feeding assays using neonate Trichoplusia ni (Hubner) for dosage response or single-dosage comparisons. Unformulated virus exposed to 68oC for 2 h showed no loss of insecticidal activity, whereas exposure to 90oC for 30 min caused >20% loss of activity. Thus, residence at higher temperatures in drying systems could adversely affect virus activity. Spray-dried formulations made with Indulin AT lignin (pH 8.5, 9.5, and 10.5) lost insecticidal activity with increasing alkalinity ...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5c1b3818dbdd141ebe895115e6a86bb8 https://doi.org/10.1080/09583150600828312 Zobrazit plný text záznamu