Improving the physiological relevance of drug testing for drug-loaded nanoparticles using 3D tumor cell cultures
| Autor: | Marzieh Mousavi, Anuja Bokare, Priya Nimbalkar, Folarin Erogbogbo, Peter Tabada, Melinda G. Simon, Jeffrey Chung |
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| Rok vydání: | 2019 |
| Předmět: |
Drug
Materials science media_common.quotation_subject Nanoparticle Vortex mixer 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences In vitro 0104 chemical sciences Cell culture Drug delivery Biophysics medicine General Materials Science Doxorubicin 0210 nano-technology Cytotoxicity medicine.drug media_common |
| Zdroj: | MRS Communications. 9:1053-1059 |
| ISSN: | 2159-6867 2159-6859 |
| DOI: | 10.1557/mrc.2019.91 |
| Popis: | Nanoparticle-mediated drug delivery has the potential to overcome several limitations of cancer chemotherapy. Lipid polymer hybrid nanoparticles (LPHNPs) have been demonstrated to exhibit superior cellular delivery efficacy. Hence, doxorubicin (a chemotherapeutic drug)- loaded LPHNPs have been synthesized by three-dimensional (3D)-printed herringbone-patterned multi-inlet vortex mixer. This method offers rapid and efficient mixing of reactants yielding controllable and reproducible synthesis of LPHNPs. The cytotoxicity of LPHNPs is tested using two-dimensional (2D) and 3D microenvironments. Results obtained from 3D cell cultures showed major differences in cytotoxicity in comparison with 2D cultures. These results have broad implications in predicting in vitro LPHNP toxicology. |
| Databáze: | OpenAIRE |
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