PEGylated cationic hybrid bicellar nanodisc for efficient siRNA delivery
| Autor: | Tie-Jun Sten Shi, Deyao Zhao, Min Chen, Yushen Jin, Xiuli Yue, Yunhui Zhao, Zicai Liang, Xiaoxia Wang, Huiqing Cao, Xiaorui Han, Renfa Liu, Xiaolong Liang, Yanyan Li, Shuquan Zheng, Yidi Wu |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Liposome Biocompatibility Chemistry General Chemical Engineering Cationic polymerization Nanotechnology 02 engineering and technology General Chemistry Transfection 021001 nanoscience & nanotechnology In vitro transfection In vitro 03 medical and health sciences 030104 developmental biology In vivo 0210 nano-technology Nanodisc |
| Zdroj: | RSC Advances. 6:113745-113753 |
| ISSN: | 2046-2069 |
| DOI: | 10.1039/c6ra24268e |
| Popis: | A hybrid cationic bicellar nanodisc was prepared by a conventional Bangham method in combination with a sol–gel reaction and self-assembly process. The relatively small size and disc-like shape was very stable because the incorporation of the organic–inorganic hybrid lipid had formed a crosslinked siloxane net structure on the surface. Physicochemical properties, biocompatibility, intracellular trafficking behavior, in vitro transfection efficiency and in vivo distribution of the bicellar nanodiscs were investigated. The unique characteristics of the cationic PEGylated hybrid bicellar nanodiscs address many of the deficiencies relating to current liposome technology. By optimizing the doping ratio of PEG-containing lipid, we found that a doping ratio of 1 mol% is enough to confer an excellent in vivo delivery performance while not compromising the transfection efficacy in vitro. The distinct disc-like shape, high stability conferred by the hybrid lipid and modest siRNA delivery performance make this platform promising as a siRNA vehicle for efficient siRNA delivery both in vitro and in vivo. |
| Databáze: | OpenAIRE |
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