Characterization of bacterial biomass potential activity by proteolytic activity measurements
| Autor: | J. C. Block, C. Bouzendorffer, F. Colin, Pascale Bauda, G. Faup |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Protease
biology Chemistry medicine.medical_treatment Bacillus cereus Biomass Substrate (chemistry) General Medicine biology.organism_classification Enterobacter aerogenes Activated sludge Biochemistry Dry weight medicine Environmental Chemistry Food science Waste Management and Disposal Bacteria Water Science and Technology |
| Zdroj: | Environmental Technology. 11:785-792 |
| ISSN: | 1479-487X 0959-3330 |
| DOI: | 10.1080/09593339009384924 |
| Popis: | Bacterial protease activity was assayed using the L Leucine s naphthylamide as artificial substrate. Apparent KM values ranging from 112 to 606 ymoles of substrate consumed /g of biomass (dry weight) were obtained with activated sludge samples. Specific activities were in the range from 7 to 26 umoles of substrate consumed/min/g of sludge (dry weight). It was established that proteolytic activity from various bacterial populations (Klebsiella aerogenes, Bacillus cereus and activated sludge) can be conveniently measured at 30°C and pH 8.5. In these conditions, the substrate and product molecules of the protease assay are non biodegradable, non toxic and not significantly adsorbed by the biomass. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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