IgG B memory cell subpopulations: differences in susceptibility to stimulation by TI-1 and TI-2 antigens
| Autor: | T V Tittle, M B Rittenberg |
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| Rok vydání: | 1980 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 124:202-206 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.124.1.202 |
| Popis: | Spleen cells from mice primed with the thymus dependent (TD) antigen trinitrophenyl keyhole limpet hemocyanin several months earlier can be stimulated in vitro to produce an IgG anti-hapten response to TD as well as thymus independent (TI) forms of the hapten. Selective killing of TD or TI-2 responding B cells can be accomplished with the corresponding antigen by bromouridine deoxyribose (BUdR) and light treatment without affecting the other population. In contrast, we show here that selective killing with TI-1 antigens does not occur. Rather, the TI-1 antigens, TNP-Brucella abortus or TNP-lipopolysaccharide, eliminate both TD and TI-2 responding IgG memory B cells. All TNP-responding B cells are similarly eliminated if cultures are challenged simultaneously with TD and TI-2 antigens before BUdR and light but not when they are challenged with either a TD or TI-2 antigen separately. We conclude that IgG memory B cell precursors stimulated to produce anti-TNP by TD or TI-2 forms of the hapten are defined by only two functionally distinct subpopulations and that TI-1 antigens can stimulate both of these populations at least to divide. |
| Databáze: | OpenAIRE |
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