Bioactive SiO2@Ru nanoparticles for osteogenic differentiation of mesenchymal stem cells via activation of Akt signaling pathways
| Autor: | Chuping Zheng, Yunfei Yu, Ning Deng, Na Huang, Jie Liu, Ying Liu |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell signaling Materials science Mesenchymal stem cell Cell Biomedical Engineering 02 engineering and technology General Chemistry General Medicine 021001 nanoscience & nanotechnology Cell biology 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology medicine.anatomical_structure chemistry medicine General Materials Science LY294002 Signal transduction 0210 nano-technology Cell adhesion Bone regeneration Protein kinase B |
| Zdroj: | Journal of Materials Chemistry B. 4:4389-4401 |
| ISSN: | 2050-7518 2050-750X |
| DOI: | 10.1039/c5tb01898f |
| Popis: | The surface chemistry of materials has an interactive influence on cell behavior. It is now well established that surface chemistry can affect cell adhesion, proliferation, and differentiation. Although amino (NH2)-terminated surfaces generated by the modification of nanoparticles with silane can promote osteogenic differentiation of mesenchymal stem cells (MSCs), how silica surfaces with ruthenium nanoparticles (SiO2@Ru) act on MSCs remains largely unknown. A concentration of 5 μg mL−1 aminopropyltriethoxysilane (APTS)-modified SiO2 nanoparticles (SiO2–NH2) or SiO2@Ru was nontoxic to MSCs, based on MTT and apoptosis assays. In addition, SiO2–NH2 and SiO2@Ru did not affect the surface phenotype or morphology of MSCs. SiO2@Ru can be used to trigger the differentiation of MSCs into osteocytes, minimising the need for exogenous biological supplementation. TEM images revealed that SiO2@Ru might interact with proteins located in the cytoplasm, which would have a further impact on subsequent cellular signaling pathways. Activation of Akt signaling pathways was observed in MSCs cultured with SiO2@Ru and these enhancement effects could be blocked by the Akt inhibitor LY294002. SiO2@Ru exhibited in vitro osteocompatibility that surpassed that of SiO2–NH2, as well as supporting the proliferation and differentiation of MSCs. This demonstrates the potential of SiO2@Ru for use in bone regeneration. |
| Databáze: | OpenAIRE |
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