Poly(pyrrole) versus poly(3,4-ethylenedioxythiophene): amperometric cholesterol biosensor matrices
| Autor: | Senem Kiralp Kayahan, Levent Toppare, Özlem Türkarslan |
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| Rok vydání: | 2008 |
| Předmět: |
Conductive polymer
Cholesterol oxidase Chemistry Substrate (chemistry) Condensed Matter Physics Electrochemistry Amperometry chemistry.chemical_compound PEDOT:PSS Organic chemistry General Materials Science Electrical and Electronic Engineering Biosensor Poly(3 4-ethylenedioxythiophene) Nuclear chemistry |
| Zdroj: | Journal of Solid State Electrochemistry. 13:657-663 |
| ISSN: | 1433-0768 1432-8488 |
| DOI: | 10.1007/s10008-008-0590-7 |
| Popis: | Two conducting polymers, poly(pyrrole) (PPy) and poly(3,4-ethylenedioxythiophene) (PEDOT) were used as immobilization matrices for cholesterol oxidase (ChOx). The amperometric responses of the enzyme electrodes were measured by monitoring oxidation current of H2O2 at +0.7 V in the absence of a mediator. Kinetic parameters, such as K m and I max, operational and storage stabilities, effects of pH and temperature were determined for both entrapment supports. K m values are found as 7.9 and 1.3 mM for PPy and PEDOT enzyme electrodes, respectively; it can be interpreted that ChOx immobilized in PEDOT shows higher affinity towards the substrate. |
| Databáze: | OpenAIRE |
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