High-resolution Chromosome Analysis in Lepidoptera

Autor:	Carll Goodpasture
Rok vydání:	1976
Předmět:	Lepidoptera genitalia biology Meiosis Chromosome analysis Insect Science Botany Trichoplusia High resolution biology.organism_classification Mitotic chromosome Molecular biology Giemsa stain Staining
Zdroj:	Annals of the Entomological Society of America. 69:764-771
ISSN:	1938-2901 0013-8746
DOI:	10.1093/aesa/69.4.764
Popis:	Improved methods for obtaining good cytological preparations from testicular tissues of Lepidoptera are described. Testes were pretreated with various hypotonic solutions, fixed in Carnoy's, and slides prepared using air dry-squash and cell suspension-air dry techniques. Air-dried slides were stained directly with Giemsa or treated with the C-band technique prior to staining. Well-spread chromosomes with good morphological characteristics were found in spermatogonial cells and spermatocytes of all stages. A detailed characterization of the mitotic chromosome complement and 1st meiotic division in males of the hesperiids Pyrgus oileus (L.), P. albescens (Plotz), and Lerema accius (Smith and Abbot) and the noctuids Trichoplusia ni (Hubner), Argyrogramma veruca (Fab.), and Prodenia ornithogalli Guenee are described.
Databáze:	OpenAIRE
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