Impedance analysis of Si/SiO2 heterostructures grafted with antibodies: an approach for immunosensor development

Autor:	Marie-Hélène Charles, Nicole Jaffrezic-Renault, H. Maupas, Bernard Mandrand, Coralie Saby, Thierry Delair, Claude Martelet, Alexey P. Soldatkin
Rok vydání:	1996
Předmět:	Analyte Reproducibility General Chemical Engineering Analytical chemistry Buffer solution Analytical Chemistry Ion selective electrode Electrochemical cell chemistry.chemical_compound Membrane chemistry Electrochemistry Selectivity Electrical impedance
Zdroj:	Journal of Electroanalytical Chemistry. 406:53-58
ISSN:	1572-6657
DOI:	10.1016/0022-0728(95)04443-4
Popis:	The feasibility of an immunosensor on the basis of impedance analysis has been studied. The measurements were performed in a classical three-electrode electrochemical cell. As a sensitive element a Si/SiO2 heterostructure was used grafted with anti-α-fetoprotein antibodies through three different coupling reagents: aminosilane, cyanosilane and polysiloxane membranes. Promising results have been obtained in such a system with polysiloxane membranes (after antigen-containing analyte incubation) through an analysis of impedance variations. The dynamic range of this sensor is between 10 and 150 ng ml−1. The results presented show good selectivity, reproducibility, and storage stability for the immunosensor, at least during 50 days of storage in PBS at +4°C.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5bdc55cd328ad4c93f1f740cc51b7648 https://doi.org/10.1016/0022-0728(95)04443-4 Zobrazit plný text záznamu Full Text from ScienceDirect