A putative 6-transmembrane nitrate transporter OsNRT1.1b plays a key role in rice under low nitrogen
Autor: | Yanling Xu, Qisong Miao, Huimin Feng, Guohua Xu, Yawen Tan, Xiaorong Fan |
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Rok vydání: | 2015 |
Předmět: |
inorganic chemicals
0106 biological sciences 0301 basic medicine biology Xenopus food and beverages Transporter Plant Science Oryza biology.organism_classification 01 natural sciences Biochemistry Genetically modified rice General Biochemistry Genetics and Molecular Biology Transmembrane protein Transport protein 03 medical and health sciences Transmembrane domain 030104 developmental biology RNA splicing 010606 plant biology & botany |
Zdroj: | Journal of Integrative Plant Biology. 58:590-599 |
ISSN: | 1672-9072 |
DOI: | 10.1111/jipb.12382 |
Popis: | OsNRT1.1a is a low-affinity nitrate (NO3 (-) ) transporter gene. In this study, another mRNA splicing product, OsNRT1.1b, putatively encoding a protein with six transmembrane domains, was identified based on the rice genomic database and bioinformatics analysis. OsNRT1.1a/OsNRT1.1b expression in Xenopus oocytes showed OsNRT1.1a-expressing oocytes accumulated (15) N levels to about half as compared to OsNRT1.1b-expressing oocytes. The electrophysiological recording of OsNRT1.1b-expressing oocytes treated with 0.25 mM NO3 (-) confirmed (15) N accumulation data. More functional assays were performed to examine the function of OsNRT1.1b in rice. The expression of both OsNRT1.1a and OsNRT1.1b was abundant in roots and downregulated by nitrogen (N) deficiency. The shoot biomass of transgenic rice plants with OsNRT1.1a or OsNRT1.1b overexpression increased under various N supplies under hydroponic conditions compared to wild-type (WT). The OsNRT1.1a overexpression lines showed increased plant N accumulation compared to the WT in 1.25 mM NH4 NO3 and 2.5 mM NO3 (-) or NH4 (+) treatments, but not in 0.125 mM NH4 NO3 . However, OsNRT1.1b overexpression lines increased total N accumulation in all N treatments, including 0.125 mM NH4 NO3 , suggesting that under low N condition, OsNRT1.1b would accumulate more N in plants and improve rice growth, but also that OsNRT1.1a had no such function in rice plants. |
Databáze: | OpenAIRE |
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