INVESTIGATING THE ROLE OF NF-κB, COX-1, COX-2, COMT, IL-10, IL-6 AND TNF-α IN MODULATING ANTI-NOCICEPTIVE ACTIVITY OF METHANOLIC EXTRACT OF ENTADA PHASEOLOIDES

Autor:	Lipika Buragohain, S. M. Abdul Aziz Barbhuiya, Acheenta Gohain Barua, Ajay Kakati, Chandana Choudhury Barua
Rok vydání:	2020
Předmět:	Pharmacology biology Chemistry medicine.medical_treatment Analgesic Pharmaceutical Science NF-κB Diclofenac Sodium Reverse transcription polymerase chain reaction Interleukin 10 chemistry.chemical_compound Pentazocine Cytokine medicine biology.protein Interleukin 6 medicine.drug
Zdroj:	International Journal of Pharmacy and Pharmaceutical Sciences. :172-179
ISSN:	0975-1491 2656-0097
DOI:	10.22159/ijpps.2020v12i8.38104
Popis:	Objective: To investigate the analgesic activity of methanolic extract of Entada phaseoloides (MEEP) along with its molecular mechanistic pathway. Methods: Swiss albino female mice and Wistar rats of either sex were administered orally with MEEP extracts (100, 200 and 400 mg/kg) and pentazocine, tramadol and diclofenac sodium, as standard drugs. Following administration, anti-nociceptive activity was evaluated using an acetic acid-induced writhing test, Eddy’s hot plate, and hot water immersion test. Serum was collected for molecular expression of various proteins and genes using Reverse Transcriptase PCR and Western Blotting. Results: Acetic acid writhing test, a frequently used method to assess peripheral analgesic activity, revealed that MEEP reduced peripherally induced pain in a dose-dependent manner. Likewise, Eddy’s hot plate and hot water immersion methods, often implicated for testing central analgesic activities, showed that MEEP is bestowed with the capability to counteract analgesia in a dose and time-dependent manner. Pro-inflammatory cytokines and factors like COX-2, IL-6, TNF-α, and NF-κB that cause inflammatory responses and pain were significantly reduced, suggesting its analgesic and anti-inflammatory potential. This analgesic and the anti-inflammatory role played by MEEP is also supported by the up-regulation of anti-inflammatory cytokine IL-10 and COMT and COX-1 enzyme demonstrated no significant difference between the groups. Conclusion: The study revealed the weak peripheral and potent central analgesic property MEEP by modulating pro-inflammatory and anti-inflammatory pathways.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5bc024e74d73e64b2a4384a801052e4a https://doi.org/10.22159/ijpps.2020v12i8.38104 Zobrazit plný text záznamu