Fabrication and Characterization of Cysteine-Functionalized Zinc Oxide Nanoparticles for Enzyme Immobilization
| Autor: | Nikhil Kumar, Reecha Sahu, Lata Sheo Bachan Upadhyay, Aditya Dutt, Nishant Verma |
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| Rok vydání: | 2017 |
| Předmět: |
Immobilized enzyme
Reducing agent Biochemistry (medical) Clinical Biochemistry Inorganic chemistry chemistry.chemical_element Nanoparticle 02 engineering and technology Zinc 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Biochemistry 0104 chemical sciences Analytical Chemistry Crystallinity chemistry Covalent bond Electrochemistry Surface modification 0210 nano-technology Spectroscopy Nuclear chemistry Cysteine |
| Zdroj: | Analytical Letters. 50:1839-1850 |
| ISSN: | 1532-236X 0003-2719 |
| Popis: | A green approach is reported for the synthesis of cysteine-functionalized zinc oxide nanoparticles using potato extract as a nontoxic and economical reducing agent. The cysteine-functionalized nanoparticles were used as a support for enzyme immobilization. The structural morphology, crystallinity, and surface functionalization were characterized by scanning electron microscopy, X-ray diffraction, and infrared spectroscopy, respectively. Spherical nanoparticles from 150 to 200 nm were used to evaluate the immobilization efficiency for urease through covalent attachment on the glutaraldehyde-activated amino group of cysteine. In comparison to the unmodified nanoparticles, 62.9% enzyme loading with 72.45% of enzyme specific activity was recovered which was 56% higher than on bare zinc oxide nanoparticles. The point of addition of cysteine during the nanoparticle synthesis had a direct effect on the immobilization efficiency. The immobilized enzyme-specific activity was reduced to 34.32% when cysteine... |
| Databáze: | OpenAIRE |
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