Autor:	Stanisław Bielecki, Richard Idem Somiari
Rok vydání:	1999
Předmět:	Gel permeation chromatography chemistry.chemical_compound Column chromatography Chromatography Invertase chemistry Ion exchange Size-exclusion chromatography Ion chromatography Butyl acetate Applied Microbiology and Biotechnology Biochemistry Amperometry
Zdroj:	Biotechnology Techniques. 13:625-629
ISSN:	0951-208X
DOI:	10.1023/a:1008912230106
Popis:	By using a two-step protocol involving flash chromatography on silica gel-60 and gel filtration on Biogel P2 we isolated 56 g pure kestose/L after enzymatic synthesis with 115 U invertase in media containing 50% (w/w) sucrose and 90% (v/v) butyl acetate. The isolated product is homogenous after high pH anion exchange chromatography with pulsed amperometric detection and is suitable for downstream utilization without further purification.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5b9d59d4b68fdff5c7abf4e511eccec4 https://doi.org/10.1023/a:1008912230106 Zobrazit plný text záznamu Full text from SpringerLink