Nicotinamide adenine dinucleotide-specific glutamate dehydrogenase of Neurospora. VI. Isolation and sequences of eighteen fragments from the cyanogen bromide digest

Autor:	Joseph F. Nyc, B M Austen, Emil L. Smith
Rok vydání:	1977
Předmět:	biology Chemistry Glutamate dehydrogenase Cell Biology Polypeptide chain Nicotinamide adenine dinucleotide biology.organism_classification Cleavage (embryo) Biochemistry Neurospora Neurospora crassa chemistry.chemical_compound Hydrolysis Cyanogen bromide Molecular Biology
Zdroj:	Journal of Biological Chemistry. 252:8160-8173
ISSN:	0021-9258
DOI:	10.1016/s0021-9258(17)40952-5
Popis:	The 1030-residue polypeptide chain of the NAD-specific glutamate dehydrogenase of Neurospora crassa was fragmented by treatment with cyanogen bromide. The isolation and sequences of 18 fragments ranging in size from 4 to 51 residues are described. Some of these peptides proved to be cleavage products resulting from hydrolysis at acid-sensitive aspartyl-prolyl bonds. Some overlaps could be deduced on the basis of known sequences of peptides obtained by tryptic hydrolysis.
Databáze:	OpenAIRE
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