Radiation-induced DNA double-strand breaks in dependence on protein concentration and under aerobic and anaerobic conditions

Autor:	H. Schüssler, G. Schwotzer, Luitpold Distel
Rok vydání:	2006
Předmět:	Radiation biology Chemistry Radical Serum albumin Electrophoresis chemistry.chemical_compound Biochemistry Oxygen enhancement ratio biology.protein Irradiation Anaerobic exercise Protein concentration DNA
Zdroj:	Radiation Physics and Chemistry. 75:210-217
ISSN:	0969-806X
DOI:	10.1016/j.radphyschem.2005.08.018
Popis:	Double-stranded DNA alone and with serum albumin in eight different weight ratios were irradiated with X-rays in phosphate buffer in air-free and aerated media. Double-strand breaks (dsb) were determined by electrophoresis. The oxygen enhancement ratio for dsb increased with increasing protein concentration from 0.8 to 3.0, presumably caused by the reaction of protein peroxyl radicals. In air-free media, serum albumin protects against radiation-induced double-strand breaking more effectively than in the presence of air, because a part of DNA radicals does not form dsb, but DNA–protein crosslinks.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5b945eb28bedefbef89c9d74e715f9e9 https://doi.org/10.1016/j.radphyschem.2005.08.018 Zobrazit plný text záznamu Full Text from ScienceDirect