W1876 Stool Protein Markers for Colorectal Cancer: Identification of High-Abundance Candidates By Mass Spectroscopy
| Autor: | David A. Ahlquist, Jessica C. Albright, Patrick S. Quint, Ann L. Oberg, Douglas W. Mahoney, Harold R. Bergen, Mary E. Devens, Jonathan J. Harrington |
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| Rok vydání: | 2009 |
| Předmět: |
Oncology
medicine.medical_specialty Hepatology business.industry Colorectal cancer Stomach Gastroenterology Chronic gastritis Intestinal metaplasia Cancer medicine.disease medicine.disease_cause medicine.anatomical_structure Internal medicine DNA methylation medicine Gastric mucosa Carcinogenesis business |
| Zdroj: | Gastroenterology. 136:A-745 |
| ISSN: | 0016-5085 |
| Popis: | Background and Aim: Altered expression of multidrug resistance 1 (MDR1) contributes to various cancers and its disease progression. DNA methylation is reported to present in various non-cancerous tissue as age-related phenomenon, suggesting that it occurs early in the process of tumorgenesis. We investigated the effect of MDR 1 gene promoter methylation on carcinogenesis in the stomach. Methods: In study A, we estimated the methylation ratio (MR) of the MDR1 gene promoter in both antral noncancerous mucosa and cancer lesions in 83 patients with gastric cancer (GC). In study B, we also estimated the MR in noncancerous gastric mucosa in 127 patients with GC and 82 cancer free (non-GC) subjects. Degree of histological chronic gastritis was scored according to updated Sydney systems in 200 subjects. We employed methylation-specific PCR method. Results: In the study A, MR of cancer lesions (31.3±20.5) was significantly higher than that of non noncancerous mucosa (20.9±31.3, p= 0.0004). MR was especially higher in Lauren's intestinal cancer (p |
| Databáze: | OpenAIRE |
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