Purification of Dengue Virus Particles by One-Step Ceramic Hydroxyapatite Chromatography

Autor:	Yae Kurosawa, Maiko Saito, Tsuneo Okuyama, Shintaro Kobayashi
Rok vydání:	2012
Předmět:	Infectivity Chromatography Hemagglutination Chemistry Elution viruses One-Step Dengue virus Japanese encephalitis medicine.disease_cause medicine.disease Virology Virus visual_art medicine visual_art.visual_art_medium Ceramic
Zdroj:	World Journal of Vaccines. :155-160
ISSN:	2160-5823 2160-5815
Popis:	Dengue virus type 2 ThNH7/93 retained infectious activity after purification by ceramic hydroxyapatite chromatogra-phy. Dengue virus type 2 culture fluid was loaded onto the ceramic hydroxyapatite column and eluted with a linear gradient of sodium phosphate buffer. Culture fluid and protein contaminants derived from host cells were eluted initially, followed by elutions of dsDNA, and then dengue virus type 2. The recoveries of dengue virus type 2 were 64 ± 14% (n = 11) in the hemagglutination (HA) test and 60% (n = 2) determined by focus assay for viral infectivity. This protocol was highly reproducible, simple, rapid, and appears applicable to other virus species such as influenza virus, Japanese encephalitis virus and adenovirus.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5b8bd10b5d424544dc38a7720aabae88 https://doi.org/10.4236/wjv.2012.23020 Zobrazit plný text záznamu