LC-MS/MS method for the determination of pitolisant: application to rat pharmacokinetic and brain penetration studies

Autor: Gopinadh Bhyrapuneni, Himabindu Vurimindi, Hanumanth Rao Pantangi, Mahesh Reddy Jonnala, Devender Reddy Ajjala, Vishwottam Kandikere, Ramakrishna Nirogi, N. R. Muddana
Rok vydání: 2013
Předmět:
Zdroj: Biomedical Chromatography. 27:1431-1437
ISSN: 0269-3879
DOI: 10.1002/bmc.2939
Popis: A simple and sensitive LC-MS/MS method was developed and validated for the quantitation of pitolisant, an H3 receptor antagonist/inverse agonist. Acetonitrile protein precipitation technique was used to prepare rat blood and brain tissue homogenate samples by using aripiprazole as internal standard (IS). Chromatographic separation was performed by using Xbridge column (2.1 × 50 mm, 3.5 µm) with a gradient elution program. The mobile phase consists of ammonium formate (10 mm) with 0.2% formic acid and acetonitrile. Multiple reaction monitoring mode was used in positive polarity with a transition of m/z 296.3 98.2 for the pitolisant and m/z 448.2 285.3 for the IS. The calibration curves were linear in the range of 0.1–100 ng/mL in both the blood and brain homogenate samples. This method was applied to quantify samples obtained from the pharmacokinetic and brain penetration studies in male wistar rats. Mean maximum concentration, area under the curve from zero to infinity and half-life of the pitolisant were found to be 3.4 ± 1.7 ng/mL, 5 ± 4 ng h/mL and 1.9 ± 0.3 h, respectively, after a 3 mg/kg oral dose. The mean calculated concentrations in the brain were found to be 38, 60 and 52 ng/g at 0.5, 1 and 2 h, respectively. Copyright © 2013 John Wiley & Sons, Ltd.
Databáze: OpenAIRE
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