Safranal Inhibits HeLa Cell Viability by Perturbing the Reassembly Potential of Microtubules
| Autor: | Sinjan Choudhary, Sanith Cheriyamundath, Manu Lopus |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pharmacology biology ved/biology ved/biology.organism_classification_rank.species biology.organism_classification Safranal HeLa 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine Tubulin Mechanism of action Biochemistry chemistry Microtubule Acetylation 030220 oncology & carcinogenesis Crocus sativus medicine biology.protein Biophysics Viability assay medicine.symptom |
| Zdroj: | Phytotherapy Research. 32:170-173 |
| ISSN: | 0951-418X |
| DOI: | 10.1002/ptr.5938 |
| Popis: | Saffron, a spice from Crocus sativus, has been known for its health benefits and medicinal properties. Safranal is a component of saffron and is known for its antioxidant and anticancer properties. In this study, we elucidated a possible tubulin-targeted antiproliferative mechanism of action of safranal. In vitro, the compound perturbed secondary structure of tubulin without altering net microtubule polymer mass. It inhibited HeLa cell viability in a concentration-dependent manner, with minimal damage to cellular microtubules. However, it strongly inhibited recovery of microtubule network after cold-induced disassembly, indicating its ability to interfere with the nucleation potential of tubulin. Further, as the acetylation pattern of the safranal-treated microtubules revealed, unlike many tubulin-targeted agents, the compound did not appear to induce persistent stabilization of microtubules. Our data shows an unusual, tubulin-targeted antiproliferative mechanism of safranal. Copyright © 2017 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text